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Self-financed students can still apply until June 30th, 2019.

Master SERP+ Programme

Analytical methods (IR and UV-Vis spectroscopies, NMR, MS)



Introduction to the standard analysis technics used for chemical analysis of compounds:

  • fundamentals of mass spectrometry and main ionization techniques & fragmentations methods,
  • UV-vis absorption and fluorescence spectroscopies, IR absorption spectroscopy, application to the characterization of molecules and to analytical chemistry,
  • Nuclear Magnetic Resonance spectroscopy: principles using the vectorial model, different interactions, application to the determination of compound structures.

Detailed content

  • Part A: Mass Spectrometry
  • Part B: Optical spectroscopies & Photophysical processes
    • I. When molecules and light meet…                        
    • II. Electronic transitions – UV-visible and fluorescence     
    • III. Rovibrational spectroscopy – IR spectroscopy                    
    • IV. Nuclear Magnetic Resonance (NMR)                 

Lab trainings

  • Mass spectrometry study on gas-phase protonated peptides         
  • Fluorimetry: Polyatomic molecules in condensed phase         
  • Infrared spectroscopy: Rotation vibration spectra of CO and HCl         
  • Nuclear Magnetic Resonance                     



  • To gain an overview on the standard methods of optical spectroscopy (IR, UV-vis, fluo, NMR): associated spectral ranges, probed molecular properties, applications in analytical chemistry.
  • To understand the fundamental photochemical processes.
  • To gain knowledge about the chemical analysis of compounds using mass spectrometry.

Recommended Books

  • Physical Chemistry, P. Atkins & J. de Paula
  • Spectrometric Identification of Organic Compounds, 7th Edition, R.M. Silverstein, F.X. Webster, D. Kiemle
  • Mass spectrometry, a textbook, J.H. Gross
  • Molecular Fluorescence: Principles and Applications, B. Valeur
  • Photochemistry, C.E. Wayne & R.P. Wayne
  • NMR spectroscopy, Harald Gunther (John Wiley & Sons)
  • Chemical Analysis: Modern Instrumentation Methods and Techniques, A. Rouessac and F. Rouessac
  • NuclearMagneticResonance, P.J.Hore
  • Understanding NMR, J.Keller

Teaching Staff

Anne Lafosse (Course Coordinator)
Debora Scuderi
Rachel Méallet-Renault
Claire Romanzin
Christie Aroulanda


Lecture: 26 h
Tutorial: 9 h
Practical courses: 15 h

Grading System

Homework: 10%          
Lab: 30%                     
Exam: 60%