The students will understand how the atomic structure of biological molecules is discovered and how this knowledge is applied in modern drug discovery. If interested in this subject - they may continue their careers in the drug development industry.
- Principles of protein structure.
- Classification of protein folds.
- Anatomy and taxonomy of nucleic acids and macromolecular complexes.
- Overview of experimental methods of structural biology: cryo-EM.
- Principles of macromolecular crystallography.
- Protein handling and crystallization.
- Phasing methods in macromolecular crystallography.
- Electron density maps and model building.
- Protein Data Bank (PDB) and model validation.
- Examples of structure-based drug design.
- Protein handling and crystallization including robotic crystallization.
- Protein crystal handling, cryoprotection and X-ray diffraction experiments.
- Electron density maps and their interpretation.
- PDB and bioinformatics tools for protein analysis.
- An exercise in structure-guided drug design.
- Protein model building.
- 5 meetings with student seminars presenting assignments from current literature with discussion.
The students will understand how the atomic structure of biological molecules is discovered and how this knowledge is applied in modern drug discovery.
- Gale Rhodes, Crystallography Made Crystal Clear, 3rd edition, Academic Press, Amsterdam 2006.
- Bernhard Rupp, Biomolecular Crystallography, Garland Science, New York 2010.
- Arthur Lesk, Introduction to Protein Science, Oxford University Press 2010.
- Carl Branden & John Tooze, Introduction to Protein Structure, 2nd edition, Garland Publishing, New York 1996.