Introduction to biology: the toolbox for biophysics
- Cell biology (From DNA to proteins, Cell architecture and motility, The Cell cycle)
- Biochemistry (The elementary bricks of life: proteins, DNA, RNA, membrane/lipids)
Biophysics and microscopies for the life sciences
- Optical microscopy: Fluorescence and fluorescent probes for biology, setups for microscopy, super-resolved microscopy techniques, applications to quantitative analysis of molecular behaviors in live cells
- Introduction to Atomic Force Microscopy: Atomic Force Microscopy (AFM): Theory and application of AFM. From cells to single molecule studies (Living cells imaging, DNA imaging, biopolymer elasticity).
- Visualization of cell’s cytoskeleton: The aim of the course is to use the immunofluorescence technique to stain components of the cell (actin filaments, tubulins and microtubules) on fixed cells.
- Bioinformatics: The aim of this course is to learn how to use the PDB website and to visualize protein structures in 3D with VMD.
- Atomic force microscopy: This practical course will teach to student how to use an AFM (imaging and force curve analysis) to study fixed and dried cells (bacteria or eukaryotic cells).
- Spectroscopic analysis of the isomerization of a GFP chromophore: The aim of this practical course is to analyze the molecular events happening in a cyan fluorescent protein upon a pH jump from 7 to 5.
- Data analysis of microscopy data [3h ON, ½ classe] Students will analyze time-resolved recordings of biological parameters. They will use in-situ calibration data to quantify cellular concentrations.
This course is an introduction to several concepts of Biophysics organized by a multidisciplinary team composed of physico-chemist, physicists and cell biologists. It will focus on microscopies and their application to biology.
Activities in small groups, research related tutorials and hands-on will help to develop critical faculties of the students.